Taken out of context, it’s kinda sounding like Aunt May has also died.
Amazing Spider-Man (Vol. 1) #32
Published: c. November, 1965?
Cover Date: January, 1966
“Man on a Rampage!”
Plot: Steve Ditko
Script: Stan Lee
Artist: Steve Ditko
Inker: Steve Ditko
Letterer: Artie Simek